The density, molar volume, apparent molar volume, viscosity and other parameters of Cu(II) surfactant derived from karanj oil in non-aqueous solvents of varying compositions have been determined at constant temperature 298.15 K. The results were used to determine the critical micelle concentration (CMC), soap-solvent interactions and the effect of chain length of the surface active molecule on various parameters. The CMC values of copper karanj soap solutions decreased with the increase in the concentration of polar solvent methanol in the solvent mixture. These studies were done to study the solution behaviour, micellar features and various interactions of the derived biologically active surfactant with polar and non-polar solvents to understand the applied part of the molecule. The apparent molar volume has been examined in terms of Masson equation, and the limiting apparent molar volume has been interpreted in terms of solute-solvent interaction. The detailed study of aforesaid compounds clearly indicates that the solutesolvent interaction decreases with the increase in ring strain and size of the synthesised complexes. The conclusions with regard to solute-solute and solute-solvent interaction have been discussed in terms of well-known Moulik’s and Jones-Dole equations. This vital information plays an important role in the selection of the synthesised molecule for various industrial and biological applications.